Abcam, ab269117, Recombinant human USP16 protein (Active)

Size: 5µg
Recombinant human USP16 protein (Active) is a Human Fragment protein, in the 187 to 823 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>85% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:GST tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab269117 was 24 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.,
Accession:Q9Y5T5,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP16 also known as Ubiquitin Specific Peptidase 16 functions as a deubiquitinating enzyme that regulates protein degradation by cleaving ubiquitin from ubiquitin-conjugated protein substrates. It has a molecular mass of approximately 97 kDa. USP16 is expressed in various tissues with high levels in the brain and heart. It has an integral role in maintaining protein homeostasis by regulating degradation pathways necessary for cellular function.
Biological function summary
The enzymatic activity of USP16 removes ubiquitin moieties contributing to the stabilization of target proteins. It participates in several cellular processes including cell cycle regulation DNA damage response and chromatin remodeling. USP16 is associated with the Polycomb group (PcG) complex which plays a role in transcriptional repression. This involvement in epigenetic regulation highlights its importance in gene expression and cellular differentiation.
Pathways
USP16 is significant in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway USP16 regulates beta-catenin degradation influencing cell proliferation and differentiation. Through the Hedgehog pathway USP16 impacts the stability of GLI transcription factors affecting developmental processes and tissue patterning. USP16 interacts with proteins like Axin and APC in the Wnt pathway illustrating its role in complex regulatory networks.
Researchers have found connections between USP16 dysregulation and cancer particularly leukemia and neurodegenerative diseases like Alzheimer's disease. In cancer USP16 overexpression can lead to increased cell proliferation through pathways involving proteins such as MYC and Cyclin D1. Related to neurodegenerative disorders USP16's interaction with proteins like amyloid precursor protein (APP) contributes to pathologies like Alzheimer's where proteostasis and ubiquitination play critical roles. Understanding USP16's functions and connections can provide insights into therapeutic targets for these diseases.