Abcam, ab269370, Human CXCL13 ELISA Kit (BCA-1)

Size: 1 x 96Tests
Human CXCL13 ELISA Kit (BCA-1) is a single-wash 90-min Simplestep used to quantify Human CXCL13 (BCA-1) with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CXCL13 ELISA Kit (BCA-1) ab269370 is a rapid single-wash 90-min Sandwich ELISA to measure Human CXCL13 (BCA-1)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, saliva, serum. This SimpleStep sensitivity is 1 pg/mL.
How the assay works
Human CXCL13 (BCA-1)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CXCL13 ELISA Kit (BCA-1) ab269370 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
CXCL13 (C-X-C motif chemokine 13), also known as BCA-1(B-lymphocyte chemoattractant/B cell-attracting chemokine 1) is a member of the CXC chemokine superfamily. CXCL13 is produced by stromal cells from the liver, spleen, lymph nodes and the gut. It binds to the CXCR5 receptor in lymphoid follicles controlling the homing signal of B cells and subsets of T cells to the lymphoid nodes, compartmentalizing lymphocytes and antigen presenting cells within the follicles of lymphoid tissues. CXCL13 is also required for the embryonic development of most of lymph nodes and Peyer's patches. CXCL13-/- as well as CXCR5-/- mice lack secondary lymphoid structures and show a disrupted splenic architecture.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CXCL13 also known as B cell-attracting chemokine 1 (BCA-1) is a chemokine with a molecular weight of approximately 11 kDa. This protein plays an important mechanical role in the immune system by guiding the movement of B cells via chemotaxis. CXCL13 expression occurs mainly in secondary lymphoid tissues such as the spleen lymph nodes and mucosa-associated lymphoid tissue. Its expression is also noted in certain non-lymphoid tissues under inflammatory conditions.
Biological function summary
In the context of immune response and lymphoid tissue organization CXCL13 attracts B cells by interacting with the chemokine receptor CXCR5. These interactions support the formation of B-cell follicles essential for efficient immune responses. CXCL13 is not known to be part of a larger protein complex but instead works through its specific receptor-ligand interactions. Its role in guiding cell migration is vital for the development of secondary lymphoid structures.
Pathways
CXCL13 is significantly involved in the B-cell receptor signaling pathway. The chemokine facilitates the homing of B cells to follicles an important step in the adaptive immune response. CXCL13 closely associates with CXCR5 its specific receptor playing a part in establishing the spatial organization of lymphoid tissues. Additionally the chemokine influences the germinal center reaction an integral part of the immune pathway for antibody maturation.
CXCL13 levels have been linked to autoimmune diseases like rheumatoid arthritis and certain types of lymphomas. In rheumatoid arthritis increased CXCL13 expression correlates with disease severity related to its role in recruiting B cells that contribute to chronic inflammation. In lymphomas particularly those involving B cells CXCL13 supports the growth and survival of malignant cells. Its association with disease states often involves changes in CXCR5 expression or function contributing to disrupted B-cell localization and abnormal immune responses.