Abcam, ab269374, Human Osteopontin ELISA Kit

Size: 1 x 96Tests
Human Osteopontin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteopontin with a sensitivity of 88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 88 pg/mL,
Range:109.38 - 7000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Osteopontin ELISA Kit ab269374 is a rapid single-wash 90-min Sandwich ELISA to measure Human Osteopontin in cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, milk, serum, urine. This SimpleStep sensitivity is 88 pg/mL.
How the assay works
Human Osteopontin SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Osteopontin ELISA Kit ab269374 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Osteopontin antibody [EPR21139-218] - BSA and Azide free (Capture)
ab269411
- Anti-Osteopontin antibody [EPR21139-316] - BSA and Azide free (Detector)
ab269411
Osteopontin (OPN) is a 35kDa secreted adhesive glycophosphoprotein expressed mainly in bone, teeth, kidney, epithelial lining tissues, activated T cells, macrophages and breast milk. It is processed by matrix metalloproteases 3 and 4 as well as thrombin. It can be present as a cytokine-like soluble protein or as an immobilized extracellular matrix protein. It is important in multiple physiologic and pathologic processes including survival, adhesion, migration, angiogenesis, apoptosis and inflammation. High levels of Osteopontin is used as a biomarker of poor prognosis in patients with breast cancer, multiple myeloma and prostate cancer. Osteopontin has a role in the immune response as a chemotactic factor for macrophages, dendritic cells and T cells. It influences both cell-mediated and humoral immunity and Th1-cytokine functions as well as enhancing B lymphocytes proliferation and immunoglogulin production.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary
Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.
Pathways
Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.
Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.