Abcam, ab269382, Anti-proANP antibody [EPR22091-19] - BSA and Azide free (Capture)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal proANP antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22091-19,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab269382 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse/Rat NT-ProANP. The recommended pair for sandwich ELISA is:
Capture: ab269382, Mouse/Rat NT-ProANP Capture Antibody (unconjugated)
Detector:
ab269383
, Mouse/Rat NT-ProANP Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml for mouse and 62.5 - 4000 pg/ml for rat.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proANP also known as N-terminal pro-atrial natriuretic peptide (NT-proANP) is a biochemical precursor involved in the synthesis of atrial natriuretic peptide (ANP). This peptide is expressed mainly in the cardiac atrial myocytes. proANP has a molecular mass of approximately 28 kDa. The heart releases it in response to atrial stretch due to increased blood volume assisting the body in managing vascular homeostasis.
Biological function summary
NT-proANP participates in fluid balance and blood pressure regulation. It is part of a complex system involved in natriuresis which promotes sodium excretion via urine therefore reducing blood volume and pressure. ANP the active hormone plays a large role in vasodilation contributing to cardiovascular health by decreasing peripheral resistance and reducing cardiac workload.
Pathways
ProANP's role extends into the renin-angiotensin-aldosterone system (RAAS) and the natriuretic peptide system. Within these pathways NT-proANP acts to counterbalance the effects of angiotensin II and aldosterone which increase blood pressure and fluid retention. It has a significant relationship with protein NPPB also known as brain natriuretic peptide (BNP) both of which function to support cardiovascular stability and electrolyte balance.
NT-proANP has connections to conditions like heart failure and hypertension. Elevated levels of NT-proANP may indicate heart failure as the heart releases more peptides in response to increased atrial pressure. NT-proANP's relationship with NPPB is clinically useful as both markers are used to diagnose and monitor heart failure. Their combined expression offers insights into cardiac function and the efficacy of therapeutic interventions.