Abcam, ab269541, Mouse Arginase 1 ELISA Kit

Size: 1 x 96Tests
Mouse Arginase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Arginase 1 with a sensitivity of 2.02 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.02 pg/mL,
Range:7.81 - 500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Arginase 1 ELISA Kit ab269541 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Arginase 1 in cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.02 pg/mL.
How the assay works
Mouse Arginase 1 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse Arginase 1 ELISA Kit ab269541 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Arginase is a key element of the urea cycle involving the conversion of L-arginine to urea and L-ornithine, which is further metabolized into metabolites that drive collagen synthesis and bioenergetic pathways critical for cell proliferation. Arginine metabolism is a critical regulator of innate and adaptive immune responses, involved in an antimicrobial effector pathway carried out by polymorphonuclear granulocytes (PMN). During PMN apoptosis, arginine is depleted in the microenvironment leading to suppressed T cell and natural killer cell proliferation and cytokine secretion. Arginase also plays a key role in human sexual response in smooth muscle tissue.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C