Abcam, ab269553, Mouse RANKL ELISA Kit (TRANCE/TNFSF11)

Size: 1 x 96Tests
Mouse RANKL ELISA Kit (TRANCE/TNFSF11) is a single-wash 90-min Simplestep used to quantify Mouse RANKL (TRANCE/TNFSF11) with a sensitivity of 1.85 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.85 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (ab269553) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TRANCE/TNFSF11) protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RANKL (TRANCE/TNFSF11) with 1.85 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Receptor activator of nuclear factor kappa-β ligand (RANKL) is a type II membrane protein and belongs to the tumor necrosis factor superfamily. RANKL is known to interact with TNFRS11B/OPG and TNFRSF11A/RANK which controls fundamental aspects of bone physiology and affects the immune system. Some of the specific functions of RANKL include essential regulator of bone remodeling, lymph node formation, and bone metastasis in cancer. ab269553 kit is developed to recognize extracellular soluble RANKL.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C