Abcam, ab269554, Human LECT2 ELISA Kit

Size: 1 x 96Tests
Human LECT2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LECT2 with a sensitivity of 0.706 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.706 ng/mL,
Range:1.56 - 100 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human LECT2 ELISA Kit (ab269554) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LECT2 protein in hep plasma, serum, cit plasma, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LECT2 with 0.706 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Leukocyte cell derived chemotaxin 2 (LECT2) is a highly conserved protein that functions with major development in liver regeneration, immune modulation, bone growth, neuronal development, cancer, and several more in pathophysiology. Studies have shown LECT2 to be a positive regulator of chondrocyte proliferation. Moreover, the liver is considered to be the source of LECT2 due to exceptionally high levels of mRNA. However, expression in these cells is extremely low or undetectable suggesting that hepatocytes secrete LECT2 instantly after production.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target 'LECT2' also known as leukocyte cell-derived chemotaxin 2 is a 16 kDa protein. It plays a role in immune response and signaling by acting as a chemotactic factor for neutrophils. LECT2 is a secreted protein primarily expressed in the liver and found in circulation in the bloodstream. It has been associated with acute-phase response and inflammation.
Biological function summary
LECT2 contributes to tissue regeneration liver function and modulation of immune responses. It is not known to be part of a larger protein complex but interacts with multiple receptor types on cell surfaces. LECT2 can inhibit mitogen-activated protein kinase (MAPK) pathway by binding to specific receptors affecting cell proliferation and survival. It also plays a role in metabolic processes.
Pathways
The LECT2 protein influences the MAPK and JNK pathways impacting cellular stress responses and inflammatory signaling. Its interactions with other proteins involve MAPK family members like p38 and ERK. These pathways regulate various cellular activities such as proliferation apoptosis and cytokine production positioning LECT2 as a modulator of cellular signaling networks.
LECT2 has strong connections to liver-related conditions such as fatty liver disease and liver fibrosis. It also associates with conditions like amyloid A kidney amyloidosis. In these diseases LECT2 interacts with inflammatory cytokines like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) which play roles in inflammation and fibrosis. These interactions can alter disease outcomes and affect therapeutic strategies targeting these inflammatory pathways.