Abcam, ab269562, Human MIP-3 alpha ELISA Kit (CCL20)

Size: 1 x 96Tests
Human MIP-3 alpha ELISA Kit (CCL20) is a single-wash 90-min Simplestep used to quantify Human MIP-3 alpha (CCL20) with a sensitivity of 5.11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.11 pg/mL,
Range:18.75 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human MIP-3 alpha ELISA Kit (CCL20) ab269562 is a rapid single-wash 90-min Sandwich ELISA to measure Human MIP-3 alpha (CCL20)in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 5.11 pg/mL.
How the assay works
Human MIP-3 alpha (CCL20)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human MIP-3 alpha ELISA Kit (CCL20) ab269562 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
MIP-3 alpha (MIP3a, CCL20) is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Its main function is to inhibit proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3 alpha is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. There are also low levels in thymus, prostate, testis, small intestine and colon. MIP-3 alpha is induced by bacterial lipopolysaccharides, TNF and IFNG/IFN-gamma. Stimulation with phorbol myristate acetate (PMA) in U-937 cell line and Bowes melanoma will induce expression. MIP-3 alpha shares 64.2%, 60.8%, 69.8%, 86.5% sequence homology with mouse, rat, cow, and monkey, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Macrophage Inflammatory Protein 3 alpha (MIP-3 alpha) also known as CCL20 is a chemokine with a molecular mass of approximately 9 kDa. It plays a role in immune response by mediating chemotaxis of lymphocytes and dendritic cells. MIP-3 alpha is mainly expressed in liver lung and lymphoid organs including the lymph nodes and appendix. Its expression can be induced during inflammation in response to signaling molecules like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1?).
Biological function summary
MIP-3 alpha contributes to the immune system by recruiting immune cells to sites of inflammation or injury. It is not part of a larger protein complex. Its main function is binding to the CCR6 receptor on target cells. This interaction mediates the migration and activation of the immune cells critical for initiating the immune response by increasing cell surface adhesion and motility. By drawing immune cells MIP-3 alpha significantly influences the body's capacity to fight infections and regulate inflammatory processes.
Pathways
MIP-3 alpha is integral to the inflammatory response and chemokine signaling pathways. It is involved in the immune system's adaptive response through its interaction with CCR6. This pathway overlaps with other chemokines such as MIP-1 and MIP-2 which also aid in immune cell recruitment. Furthermore MIP-3 alpha’s engagement in these pathways facilitates intercellular communication during immune responses pivotal for maintaining homeostasis and immune surveillance.
MIP-3 alpha has associations with inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. During these conditions elevated levels of MIP-3 alpha contribute to the recruitment of inflammatory cells which exacerbate symptoms. MIP-3 alpha also connects with other proteins such as TNF-alpha in these disease contexts enhancing inflammation and progression of these disorders. Targeting MIP-3 alpha and its associated pathways may provide therapeutic opportunities for alleviating these chronic inflammatory conditions.