Abcam, ab269998, Anti-UPF3B/RENT3B + UPF3A antibody [EPR24112-81]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal REN3A antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Zebrafish samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24112-81,
Isotype:IgG,
Carrier free:No,
Reacts with:Zebrafish, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody cross-reacts with zebrafish Upf3b.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UPF3B also known as RENT3B is a component of the nonsense-mediated mRNA decay (NMD) pathway with a mass of approximately 48 kDa. UPF3A is a closely related homolog sharing several functional characteristics. UPF3B is expressed in various tissues including the brain. This protein primarily functions in the nucleus and cytoplasm where it binds to mRNA and interacts with other NMD factors to facilitate the degradation of faulty transcripts. By tagging mRNA with premature stop codons for degradation UPF3B helps maintain the integrity of gene expression.
Biological function summary
UPF3B ensures accuracy in post-transcriptional regulation. Both UPF3B and UPF3A are part of the exon-junction complex (EJC) playing a role in recognizing erroneous transcripts. They work in conjunction with other core NMD proteins such as UPF1 and UPF2 to target mRNAs with errors. This mechanism is essential in protecting cells from potential damage caused by erroneous or incomplete protein products impacting gene expression at the mRNA level.
Pathways
UPF3B and UPF3A play important roles in the nonsense-mediated mRNA decay pathway which is a critical mRNA surveillance mechanism. Their interaction with UPF1 an important factor in NMD illustrates their central position in this pathway. UPF3B's role in regulating mRNA stability also links it to the broader RNA processing and degradation pathways including those involved in general mRNA turnover and quality control ensuring cellular homeostasis.
UPF3B associations relate to X-linked intellectual disabilities and neurodevelopmental disorders. Mutations in the UPF3B gene disrupt normal NMD function leading to cognitive impairments. This disruption often involves UPF1 and other NMD components. Such genetic defects highlight the importance of robust mRNA surveillance systems in neurological development and health. Furthermore the impaired function of UPF3B in this context reveals insights into the molecular basis of certain intellectual disabilities.