Abcam, ab270204, Human Neutrophil Elastase ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human Neutrophil Elastase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Neutrophil Elastase with a sensitivity of 15.8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 15.8 pg/mL,
Range:47 - 3000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Neutrophil Elastase ELISA Kit ab270204 is a rapid single-wash 90-min ELISA kit to measure Human Neutrophil Elastase cell culture media, cell Lysate, serum and plasma. 15.8 pg/mL.
How the assay works
Human Neutrophil Elastase SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Neutrophil Elastase protein in serum, plasmas, cell culture supernatant, and cell extract samples only. Urine, saliva, and milk samples have not been tested with this kit.
Human Neutrophil Elastase ELISA Kit ab270204 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Neutrophil Elastase ELISA Kit ab270204
Human Neutrophil Elastase ELISA Kit ab270204 has been used to study the plasma proteome of long-covid patients
and to study interactions of neutrophil elastase with anti-thrombin in venous thromboembolism
References:
Cristiana Iosef et al. 2023, PMID: 37301958,
Laura E Vincent et al. 2022, PMID: 35731524
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Neutrophil Elastase Antibody Pair - BSA and Azide free
ab253304
- Anti-Neutrophil Elastase antibody [EPR22896-31] - BSA and Azide free (Capture)
ab272279
- Anti-Neutrophil Elastase antibody [EPR22896-78] - BSA and Azide free (Detector)
ab272280
Related and recommended products
Human Neutrophil elastase is commonly used to study inflammation.
To measure Neutrophil elastase activity:
- Neutrophil Elastase Activity Assay Kit (Fluorometric)
ab204730
Neutrophil Elastase, also known as bone marrow serine protease, Elastase-2, human leukocyte Elastase, and medullasin is a serine protease in the peptidase S1 family and is encoded by the *ELANE* gene. Neutrophil Elastase is expressed as a zymogen by neutrophils and macrophages during inflammation, where it breaks down bacterial and host membrane proteins. Neutrophil Elastase can localize to Neutrophil extracellular traps (NETs) by the protein’s affinity for DNA.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
Biological function summary
The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.
Pathways
This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.
Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.