Abcam, ab270218, Human IL-7 ELISA Kit

Size: 1 x 96Tests / 10 x 96Tests
Human IL-7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-7 with a sensitivity of 5.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.9 pg/mL,
Range:18.8 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-7 ELISA Kit ab270218 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-7 in cell culture media, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 5.9 pg/mL.
How the assay works
Human IL-7 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IL-7 ELISA Kit ab270218 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-7 antibody [EPR19464-214] - BSA and Azide free (Capture)
ab271310
- Anti-IL-7 antibody [EPR21706-126] - BSA and Azide free (Detector)
ab271310
IL-7 (Interleukin 7) is a 20kDa hematopoietic growth factor secreted by stromal cells residing in the bone marrow, thymus, spleen and gut. IL-7 plays a critical role during early T-cell development of naпve and memory CD8+ T cells, as well as during the proliferation and differentiation of B cells. Furthermore, IL-7 has been shown to play a key role in coreceptor reversal, a process which induces the expression of CD8 receptor in a T-cell that has begun differentiation as a CD4+ T cell. IL-7 belongs to a family of short-chain four-helical bundle cytokines. These family members signal through heterodimeric receptor complexes that contain the common cytokine receptor gamma-chain, and activate signaling pathways that promote cell proliferation and survival such as the PI3K, MAPK, and JAK/STAT. IL-7 has been implicated in the development of rheumatoid arthritis (RA), HIV, acute graft-vs-host disease (aGVHD) and X-linked severe combined immunodeficiency (XSCID). In RA, IL-7 activates the production of TNF-alpha by CD4+ T cells and macrophages within the synovial fluid, leading to the inflammation characteristic of RA. In patients with HIV, IL-7 homeostasis is impaired due to decreased expression of the IL-7R. The loss of T-cell homeostasis induces the lymphopenic condition characteristic of an HIV infection. Furthermore, the T-cell cytotoxic regimen prior to bone marrow transplantation induces IL-7 secretion, stimulating growth of residual allo-reactive T cells. These allo-reactive T-cells recognize and attack the transplanted cells and host tissues which lead to the development of aGVHD. Additionally, IL-7 knockout studies in mice have demonstrated that IL-7 is crucial for T cell survival as absence of IL-7 leads to XSCID. The rodent IL-7 proteins have low homology to human IL-7; only 56-57% amino acids sequence identity.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary
IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naпve and memory T cells important for adaptive immunity.
Pathways
IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.
IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.