Abcam, ab270476, Human PPP2R5D knockout A-431 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP2R5D KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 7 bp deletion Frameshift = 99.91%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A-431,
Species or organism:Human,
Tissue:Skin,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 7 bp deletion Frameshift = 99.91%,
Disease:Epidermoid Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP2R5D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP2R5D also known as B56Δ forms part of the regulatory subunit of protein phosphatase 2A (PP2A). Protein phosphatase 2A is an important enzyme in cellular signaling that dephosphorylates target proteins. The approximate molecular mass of PPP2R5D is 72 kDa. It is found expressed in various tissues throughout the human body but its levels can vary between different cell types.
Biological function summary
PPP2R5D plays an essential role in the regulation of cell growth and division. This protein is part of the PP2A holoenzyme complex which assembles with catalytic and scaffolding subunits. Through its regulatory influence PPP2R5D affects the phosphorylation state of several substrates thereby impacting cellular processes like cell cycle progression and apoptosis.
Pathways
PPP2R5D significantly contributes to the MAPK signaling pathway and the Wnt pathway. It modulates phosphorylation states affecting signal transduction mechanisms. In the MAPK pathway PPP2R5D interacts with proteins like ERK1/2 by influencing their activity. Similarly in the Wnt pathway it acts on components important for maintaining cellular balance and signaling fidelity.
PPP2R5D mutations and dysregulation have been linked to intellectual disabilities and cancer. Changes in its function are associated with Autism Spectrum Disorder where it potentially interacts with other proteins involved in neuronal development. In cancer altered PPP2R5D activity may disrupt normal cellular growth control often involving proteins like c-Myc that are critical for proliferation and survival.