Abcam, ab270551, ATPase Assay Kit

Size: 2 x 96Tests
ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. We recommend ATPase Assay Kit ab234055 as a replacement for this kit.
Key facts
Detection method:Colorimetric,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate

Product details:
We recommend ATPase Assay Kit
ab234055
as a replacement for this kit. The assay mechanism in
ab234055
is identical to this assay kit; it is formulated with less assay components for greater convenience, and includes an ATPase enzyme positive control.
ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock™ gives a stable end-point signal and is not prone to precipitation. PiColorLock™ has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.
NB: For samples with free Pi, please check the Inorganic Phosphate Binding Resin (
ab270547
Other Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols