Abcam, ab270603, Anti-HA Affinity Resin - Amintra

Size: 1mL / 5mL
Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 6 - 8Preservative: 0.02% Sodium azideConstituents: 50% Agarose beads, 49.98% PBS

Product details:
Anti-HA Affinity Resin - Amintra (ab270603) is designed for simple, rapid HA-tagged recombinant protein purification and immunoprecipitation.
The HA tag is derived from the human influenza virus HA protein corresponding to amino acids 98-106 (YPYDVPDYA). The HA tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein, so it has been extensively used as a general epitope tag at the N-terminal or C-terminal of the fusion protein. Anti-HA Affinity Resin - Amintra is based on a 4% agarose beads matrix, and non-specific binding is rare. It can be used for HA fusion protein purification and immunoprecipitation.
The characteristics of the resin:
Description
Matrix
4% agarose beads
Ligand
Anti-HA Antibody
Binding capacity
>1mg HA fusion protein/mL medium
Particle Size (μm)
45–165
Max. Pressure
0.1 MPa, 1 bar
Storage buffer
1 x PBS with 0.02% sodium azide
Storage Temperature
For the purification of HA-tagged fusion proteins, Amintra Anti-HA Affinity Beads can be used in column chromatography, batch format and immunoprecipitation.
For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract.
When a small amount of sample is being purified, immunoprecipitation procedure is recommended.
Notes:
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Anti-HA Affinity Beads. Expedeon product codes AHA0001 (1 mL medium) and AHA0005 (5 mL medium) are the same as the current sizes: 1 mL and 5 mL, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze