Abcam, ab271355, Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HDAC4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19010-77,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human, Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab271355 is the carrier-free version of
ab195241
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC4 HDAC5 and HDAC9 are types of histone deacetylases abbreviated as HDACs. These enzymes remove acetyl groups from histone and non-histone proteins affecting gene expression by changing chromatin structure. HDAC4 has a molecular weight of about 140 kDa. HDAC4 and HDAC5 are highly expressed in the brain heart and skeletal muscle while HDAC9 is more abundant in the heart and skeletal muscle. They act as transcriptional repressors by interacting with various corepressor complexes.
Biological function summary
Histone deacetylases 4 5 and 9 influence gene expression and cellular differentiation. These proteins often form part of larger corepressor complexes such as the N-CoR and SMRT complexes allowing them to modulate transcriptional activity. They play significant roles in muscle differentiation and neural development interacting with transcription factors like MEF2 to regulate various genes involved in these processes.
Pathways
Histone deacetylases like HDAC4 5 and 9 are critical components of signaling pathways related to calcium/calmodulin-dependent calcineurin signal transduction. This pathway influences muscle development and function where they interact with MEF2 family transcription factors to control gene expression. Additionally they are involved in the regulation of the circadian rhythm through their role in the molecular clockwork partnering with proteins like CLOCK and BMAL1.
Alterations in HDAC4 5 and 9 levels or activity have connections to cardiovascular diseases and certain cancers. Dysfunctional HDAC activity can lead to hypertrophy in cardiac cells affecting heart function. Cancer progression often involves aberrant histone deacetylation with HDACs modulating oncogenic transcription factors. These HDACs closely interact with cancer-related proteins like MYC and p53 influencing tumor suppression or progression depending on cellular context.