Abcam, ab271460, Recombinant human p35 + CDK5 protein (Tagged)

Size: 10µg
Recombinant human p35 + CDK5 protein (Tagged) is a Human Full Length protein, in the 1 to 307 aa range, expressed in Baculovirus infected Sf9 cells, with >60%, suitable for SDS-PAGE.
Key facts
Purity:>60% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific acivity: 180 pmol/min/μgAssay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min.,
Accession:Q00535,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P35 is an important activator protein for Cyclin-dependent kinase 5 (CDK5) also referred to as CDK5R1. CDK5 is not a typical cyclin-dependent kinase as it does not associate with cyclins. Instead it pairs with p35 to gain its kinase activity. CDK5/p35 complex plays essential roles in various cellular processes. The molecular mass of p35 is approximately 33.5 kDa and it is expressed prominently in the central nervous system especially in the brain. This expression pattern supports its critical involvement in neuronal activities.
Biological function summary
The CDK5/p35 complex regulates processes important for neuronal function and development. It is involved in the regulation of neuronal migration differentiation and axonal guidance. This protein complex is active during brain development where it directs cytoskeletal arrangement and synaptic signaling. p35 itself also regulates the survival of neurons preventing apoptosis under normal physiological conditions. The importance of this complex extends beyond neurons influencing several other tissues but is most noted in neurobiology.
Pathways
The functions of CDK5 and p35 integrate significantly into the neurodevelopmental processes. CDK5/p35 participates in the signaling pathways involving cytoskeletal proteins like tau affecting the microtubule dynamics critical for neuron structure maintenance. Additionally the complex influences the apoptotic pathways by interacting with proteins such as bcl-2 to ensure neuron survival. These interactions highlight its role in pathways that are vital for the integrity of neuronal architecture and stability.
Improper activity of CDK5/p35 links to neurodegenerative diseases like Alzheimer's disease. Phosphorylation of tau by CDK5/p35 results in tau aggregation a pathological hallmark of Alzheimer's. Moreover alterations in the CDK5/p35 activity contribute to Parkinson's disease where dysregulation leads to neuronal death. In these contexts proteins such as beta-amyloid and alpha-synuclein also engage with CDK5 highlighting the complex's involvement in the progression of these debilitating conditions.