Abcam, ab271713, Recombinant human PHF8 protein (Active)

Size: 20µg
Recombinant human PHF8 protein (Active) is a Human Full Length protein, in the 2 to 1060 aa range, expressed in Baculovirus infected Sf9 cells, with >60%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>60% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:DDDDK tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 0.013 pmol/min/μg.,
Accession:Q9UPP1,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHF8 also known as Plant Homeodomains Finger Protein 8 is a histone demethylase enzyme. It specifically removes methyl groups from histone H3 at lysine 9 (H3K9me2/me1) and at lysine 27 (H3K27me2/me1). PHF8 is a protein with a molecular mass of around 109 kDa. This protein is expressed widely in various tissues appearing notably in brain tissues and the testis which suggests a broad physiological role.
Biological function summary
PHF8 plays an important role in the regulation of gene expression through chromatin remodeling. It forms part of transcriptional complexes and directly interacts with transcription factors and chromatin proteins. Its activity modulates gene silencing and activation processes impacting multiple cellular functions such as cell cycle progression and neurodevelopment. The activities controlled by PHF8 make it critical for maintaining normal cellular function.
Pathways
PHF8 takes part in integral signaling and epigenetic regulation pathways. It is involved in the MAPK/ERK signaling pathway which affects cell proliferation and differentiation. PHF8 also connects with other proteins such as p53 influencing pathways that govern cell growth and apoptosis. Through these interactions PHF8 helps maintain proper balance in cell cycle and development processes.
Mutations or dysregulation of PHF8 have links to developmental disorders and certain cancers. Specifically PHF8 mutations have associations with X-linked mental retardation and cleft lip/palate conditions. Dysregulated PHF8 activity interacts with proteins like p53 to influence cancer progression particularly in prostate cancer. Understanding PHF8's role aids in the exploration of therapeutic targets for these conditions.