Abcam, ab271748, Recombinant human SGPL1 protein (Active)

Size: 10µg
Recombinant human SGPL1 protein (Active) is a Human Fragment protein, in the 59 to 568 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Active,
Accession:O95470,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 1.7% ImidazoleConstituents: Methionine, PLP, 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.79% Tris HCl, 0.03% TCEP HCl

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SGPL1 also known as sphingosine-1-phosphate lyase 1 is an enzyme involved in the irreversible breakdown of sphingosine-1-phosphate (S1P). This enzyme has a molecular weight of about 63 kDa. SGPL1 is generally found in the endoplasmic reticulum of cells and is broadly expressed in various tissues including the liver kidney and brain. The key role of SGPL1 is to catalyze the cleavage of S1P an important sphingolipid signaling molecule into phosphoethanolamine and hexadecenal.
Biological function summary
Sphingosine-1-phosphate lyase 1 plays a role in maintaining the balance of sphingolipid metabolites by regulating S1P levels. This protein does not form a known complex but acts alone to facilitate the degradation process. By regulating S1P SGPL1 indirectly influences cell growth survival migration and apoptosis which are processes essential for normal cellular function. The regulation affects numerous cellular systems such as immune response and vascular function.
Pathways
Sphingosine-1-phosphate lyase 1 is integral to the sphingolipid metabolism pathway and the S1P signaling pathway. It directly breaks down S1P therefore diminishing its signaling abilities which are mediated by its interaction with S1P receptors. SGPL1 acts in the final step of sphingolipid degradation and it relates to proteins like sphingosine kinase which phosphorylates sphingosine into S1P therefore connecting two critical steps in the sphingolipid pathway.
Sphingosine-1-phosphate lyase 1 has links to conditions such as nephrotic syndrome and primary immunodeficiency. Mutations in the SGPL1 gene can disrupt sphingolipid balance leading to these diseases. Through these conditions SGPL1 relates to proteins like nephrin which are involved in kidney function and are affected in nephrotic syndrome. Understanding SGPL1's function provides insights into potential therapeutic targets for treating these disorders.