Abcam, ab271996, Anti-TROP2 antibody [EPR20043] - BSA and Azide free

Size: 100µg / 1mg
Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TROP2 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EPR20043 is the most cited clone to TROP2 - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20043,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Mouse, Human,
Applications:WB, Flow Cyt (Intra), IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of TROP2?
Anti-TROP2 [EPR20043] - BSA and Azide free (ab271996) specifically detects a band for TROP2 (UniProt: P09758) at a molecular weight of 36kDa.
Specificity confirmed
The specificity of Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996) has been confirmed by Western blot testing in TACSTD2 Knockout MCF7 cell line.
Other related products
We have a range of other formats of antibody clone [EPR20043] also available for your convenience:
ab214488
, Carrier free -
ab222935
, Carrier free - ab271996, Alexa Fluor® 647 -
ab281582
, Alexa Fluor® 488 -
ab281933
, PE -
ab303028
, APC -
ab303029
, HRP -
ab303030
, Alkaline Phosphatase -
ab308661
, Alexa Fluor® 594 -
ab310365
, Alexa Fluor® 568 -
ab312357
, Alexa Fluor® 750 -
ab321379
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.
Biological function summary
TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.
Pathways
TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.
TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.