Abcam, ab272188, Streptavidin Atto 542 Monovalent Antibody Labeling Kit

Size: 25µg / 50µg
Streptavidin Atto 542 (Monovalent) Antibody Labeling Kit (ab272188) provides a rapid, robust, and flexible method for attaching fluorochromes to biotinylated antibodies. It offers several standout features: - Enables multiplexing with multiple biotinylated antibodies - No spillover of Streptavidin Atto 542 (Monovalent) reagent between antibodies - Compatible with antibodies of the same animal host species
Product details:
Streptavidin Atto 542 Monovalent Antibody Labeling Kit ab272188 is used to label biotinylated antibodies with Atto 542.
Atto 542 is very bright and can be used as an alternative to Cy3
The Antibody - Streptavidin Atto 542 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.
Use this kit to:
- label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody
- label two or more biotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment
*multiple kits required, see list below
- label as little as 0.1 μg of biotinylated antibody in less than 5 mins without washes
Technical notes
The 50 μg size of this kit is typically sufficient to label 50 μg of antibody, the 25 μg size is sufficient to label 25 μg of antibody.
When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.
The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.
List of available monovalent streptavidin antibody labeling kits
Streptavidin Alexa Fluor
488 Equivalent (
ab272187
Streptavidin Atto 542 (ab272188) – this kit
Streptavidin Alexa Fluor
ab272189
Streptavidin Alexa Fluor
647 Equivalent (
ab272190
Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C