Abcam, ab272193, Human Semaphorin 7A ELISA Kit

Size: 1 x 96Tests
Human Semaphorin 7A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Semaphorin 7A with a sensitivity of 24.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 24.7 pg/mL,
Range:70.31 - 4500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Semaphorin 7A ELISA kit (ab272193) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Semaphorin 7A protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Semaphorin 7A with 24.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Semaphorins constitute a large class of soluble and membrane bound proteins that are known to be involved in critical immune responses and serve neuronal functions. Semaphorin 7A, also known as CD108, is a glycosylphosphatidylinositol-anchored semaphorin that promotes axon outgrowth through beta1-integrin receptors and contributes to the formation of the lateral olfactory tract. Promotes attachment, spreading and dendrite outgrowth in melanocytes and plays an important role in modulating inflammation and T-cell mediated immune response. Mouse, rat. cow and monkey share 89, 89%, 86% and 99% sequence homology, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Semaphorin 7a also known as SEMA7A or CD108 is a glycoprotein that has a molecular mass of approximately 80 kDa. This target plays a role in axonal guidance and immune response regulation. It is a member of the semaphorin family which generally acts as signaling molecules. Semaphorin 7a is expressed in various tissues including the immune system nervous system and some epithelial cells. Its expression is especially significant in T-cells and is involved in modulating cell adhesion and migration.
Biological function summary
Semaphorin 7a is implicated in diverse processes such as immune system modulation and nervous system development. It functions as part of a complex involving integrins which mediate cell-to-cell communication and interaction with the extracellular matrix. This protein can bridge the immune and neural signaling pathways indicating its multifunctional nature within organisms. Its interaction with β1 integrins enhances T-cell activation and cytokine production which is important for immune response.
Pathways
Semaphorin 7a integrates into the MAPK and PI3K signaling pathways. These pathways are essential for cell survival migration and apoptosis. In the MAPK pathway Semaphorin 7a impacts downstream signaling through its interaction with ERK proteins. Its role in the PI3K pathway connects it to AKT proteins influencing cell growth and metabolic processes. These connections show its versatility and critical role in signal transduction linked to cellular behaviors.
Semaphorin 7a has been associated with multiple sclerosis and melanoma. In multiple sclerosis altered expression of this protein can affect immune response and neural repair processes. Its connection to melanoma is linked with its role in regulating cell motility and adhesion impacting cancer progression and metastasis. Semaphorin 7a's interactions with proteins like Melanocortin 1 Receptor (MC1R) in melanoma provide insights into therapeutic targets for disease interventions.