Abcam, ab272395, Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GPCR GPR64 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23658-2,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab272395 is the carrier-free version of
ab272390
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GPCR GPR64 also known as ADGRG2 is a member of the adhesion G protein-coupled receptor family. It is characterized by large extracellular domains facilitating cell adhesion and signaling. This receptor has a mass of approximately 98 kDa. GPR64 is mainly expressed in the epididymis and plays an important role in reproductive tissues. Moreover it displays a basal activity that implicates several signaling pathways.
Biological function summary
GPCR GPR64 functions as a mediator of reproductive tract development. It is not currently known to be part of any larger complex. However its signaling contributes to the modulation of fluid reabsorption in the epididymis affecting sperm maturation and storage. Beyond the reproductive system some studies suggest roles in other tissues like osteoblasts and kidney cells.
Pathways
Several pieces of research have tied GPCR GPR64 to the cAMP signaling pathway influencing the regulation of epithelial sodium channels. This impacts ion transport and homeostasis within the reproductive tissues. Additionally other proteins such as epithelial sodium channel (ENaC) have shown interactions with GPR64 affecting fluid dynamics. Further research is exploring potential connections to the Wnt signaling pathway due to similar functional roles.
GPCR GPR64 has been linked to disorders affecting fertility such as reduced sperm motility leading to male infertility. Also abnormal expression patterns have been observed in some cancer types like prostate cancer. The interaction with ENaC makes it particularly interesting in understanding how dysregulation might impact these conditions on a cellular level providing insights for potential therapeutic targets.