Abcam, ab272397, Anti-CRF antibody [EPR23255-162] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CRF antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23255-162,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab272397 is the carrier-free version of
ab272391
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Corticotropin-releasing factor (CRF) also known as corticotropin-releasing hormone (CRH) is a peptide hormone involved in the stress response. This protein with a mass of approximately 41 kDa is predominantly produced in the hypothalamus and later secreted into the bloodstream. CRF plays an important role in activating the hypothalamic-pituitary-adrenal (HPA) axis stimulating the secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland. Its expression extends beyond the hypothalamus being present in peripheral tissues such as the adrenal glands and gastrointestinal tract.
Biological function summary
Corticotropin-releasing factor functions as a central coordinator of the body's response to stress. CRF engages several receptors including CRF receptors type 1 and 2 which mediate its actions. It forms part of a larger complex involving several other binding proteins that regulate its availability and receptor affinity. By interacting with these receptors CRF modulates diverse physiological processes such as immune responses and energy balance ensuring the maintenance of homeostasis under stress conditions.
Pathways
Corticotropin-releasing factor orchestrates important signaling within the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathways. CRF is pivotal in the activation of various signaling cascades that influence the production of glucocorticoids like cortisol. Within these pathways CRF interacts closely with proteins such as ACTH which acts downstream of CRF to potentiate adrenal corticosteroid synthesis. Another related cascade involves CRF in neuroendocrine signaling affecting neurotransmitter release and influencing behaviors related to stress and anxiety.
Corticotropin-releasing factor is heavily implicated in depression and anxiety disorders. Alterations in CRF expression and receptor sensitivity have been associated with the pathophysiology of these conditions. For instance abnormal CRF signaling can lead to elevated stress hormone levels contributing to mood disorders. CRF also connects to proteins like the glucocorticoid receptor influencing how the body processes stress-related hormones and impacting the development and progression of stress-related psychiatric disorders.