Abcam, ab272504, Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus

Size: 100µg
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) is a rabbit polyclonal antibody detecting SARS-CoV-2 Spike Glycoprotein in Western Blot, IHC-P, ICC/IF, ELISA . Suitable for SARS-CoV-2 . - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:WB, ELISA, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The immunogen for ab272504 is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa). Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins). ab272504 can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL.

Product details:
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IHC-P and WB.
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) was first used in a scientific publication in 2020 and has been cited over 48 times in peer reviewed journals. It's performance in IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) has high sensitivity and specificity.
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) has 5 independent reviews from customers.
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) specifically detects SARS-CoV-2 Spike Glycoprotein (UniProt ID: P0DTC2; Molecular weight: 140kDa) and is sold in 100 ug selling sizes.
Reagent has more than 76 citations. SARS-CoV-2 Spike Glycoprotein antibody is essential for detecting and studying the spike protein, aiding in COVID-19 research, vaccine development and therapeutic studies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 Spike Glycoprotein also known as the COVID spike protein is an important component of the virus responsible for COVID-19. This protein has a molecular weight of approximately 180 kDa and is found on the surface of the virus. It facilitates viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor. The spike protein consists of two subunits S1 and S2 where S1 contains the receptor-binding domain (RBD) responsible for engaging with ACE2 while S2 mediates viral and host membrane fusion.
Biological function summary
The spike protein serves as a primary target for the host immune response. It is part of the viral envelope complex essential for the virus's infectivity. The spike protein undergoes structural changes during host cell entry transitioning from a prefusion to a postfusion conformation. This ability to change structure is important for facilitating membrane fusion. Anti-spike antibodies such as those found in convalescent plasma and generated by vaccines target this protein aiming to neutralize the virus and prevent infection.
Pathways
The spike glycoprotein is integral to the viral infection and replication process. The renin-angiotensin system (RAS) pathway plays a significant role as ACE2 a critical component is the entry point for the virus. The spike protein's interaction with ACE2 disrupts the RAS balance leading to potential downstream effects. Additionally the spike protein's role in membrane fusion links it to the endosomal sorting complex pathways necessary for the trafficking of viral particles within host cells.
The spike protein's association with COVID-19 makes it central in understanding disease progression and potential complications. Its interaction with ACE2 is not only significant for viral entry but also links to severe acute respiratory syndrome (SARS) as both viruses exploit this receptor for entry. Moreover the association with inflammatory-related pathways indicates the spike protein's potential in driving cytokine storms which are severe immune responses contributing to the disease’s morbidity. Anti-spike antibodies and therapies are designed to mitigate these effects emphasizing the protein's importance in therapeutic strategies.