Abcam, ab272771, Human FVIII ELISA kit (total FVIII antigen)

Size: 1 x 96Tests
Human FVIII ELISA kit (total FVIII antigen) is a Sandwich ELISA used to quantify Human FVIII (total FVIII antigen) with a sensitivity of 0.00024 IU/mL - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 0.0016 - 0.84 IU/mL
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, EDTA Plasma,
Reacts with:Human,
Assay type:Quantitative,
Range:0.0016 - 0.84 IU/mL,
Assay Platform:Microplate

Product details:
Human FVIII ELISA kit (total FVIII antigen) ab272771 is a sandwich ELISA kit for the quantitative measurement of FVIII total antigen with a sensitivity of 0.00024 IU/mL.
How the assay works
Human FVIII ELISA kit ab272771 uses a pair of antibodies each specific of different epitopes at the target of interest : the capture antibody is coated on the surface of the multi-well plate to facilitate the immobilization of the antigen. The other antibody is conjugated and facilitates the detection of the antigen.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Human FVIII ELISA kit (total FVIII antigen) ab272771 protocol summary
1. Add standard or sample to appropriate wells and shake plate at 300 rpm for 30 mins
2. Wash
3. Add primary antibody to each well and shake plate at 300 rpm for 30 mins
4. Wash
5. Add Streptavidin-HRP to each well and shake plate at 300 rpm for 30 mins
6. Wash
7. Add TMB substrate to each well and shake the plate for 2-10 min
8.Add Stop solution and read at 450nm.
How other researchers are using Human FVIII ELISA kit (total FVIII antigen) ab272771
Human FVIII ELISA kit (total FVIII antigen) ab272771 has been used to determine the efficiency of FVIII-secreting transgenic mesenchymal stem cells in correcting FVIII levels in an Hemophilia A model
References
C Hemsinlioglu et al. PMID: 37022209
Related and Recommended products
Human FVIII ELISA kit (total FVIII antigen) ab272771 is commonly used to study the coagulation cascade. Other kits used to study heamostasis are :
- Human Factor IX/PTC ELISA kit
ab300307
- Factor VIIIa Activity Assay Kit (Fluorometric)
ab204696
- Human Thrombin ELISA kit
ab270210
- Human Tissue Factor ELISA kit
ab220653
- Human Factor X ELISA Kit Human Factor X ELISA Kit
- Human Factor VII ELISA Kit
ab190810

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor VIII also known as anti-hemophilic factor is a glycoprotein with a molecular mass of approximately 250 kDa. This protein circulates in the plasma mainly in the liver where hepatocytes produce it. Factor VIII remains inactive while circulating in the blood and exists within the factor VIII/von Willebrand factor complex. Upon activation it plays a significant role in the blood coagulation process.
Biological function summary
Factor VIII greatly contributes to hemostasis by serving as a cofactor for factor IXa forming the intrinsic tenase complex. This activation results in the conversion of factor X to factor Xa which is important for the common pathway of the coagulation cascade. By participating in the clotting process factor VIII ensures proper wound healing and prevention of blood loss.
Pathways
Factor VIII activity is pivotal within the coagulation cascade particularly the intrinsic and common pathways. Activation of the intrinsic pathway begins through interactions with factor IXa leading to efficient activation of factor X. This process closely relates factor VIII to proteins like factor IX and factor X ensuring proper clot formation and stabilization. Factor VIII products and assays are available to study and characterize its critical role in these pathways.
Factor VIII is closely associated with hemophilia A a genetic bleeding disorder resulting from deficiency or dysfunction of this protein. Patients with hemophilia A exhibit prolonged bleeding times due to impaired coagulation. The disorder links directly to von Willebrand factor as it protects factor VIII from degradation in circulation stabilizing its presence in the blood. Anti-factor VIII antibodies are important tools for diagnosing and studying factor VIII-related disorders.