Abcam, ab273205, Porcine IL-4 ELISA Kit

Size: 1 x 96Tests
Porcine IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine IL-4 in Pig in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Pig,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.5 pg/mL,
Range:1.638 - 400 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Porcine IL-4 ELISA Kit (ab273205) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-4 in serum (Porcine IL-4 concentration is low in normal serum/plasma and may not be detectable in this assay), plasma, and cell culture supernatants.
This assay employs an antibody specific for porcine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti porcine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C