Abcam, ab273293, Phenolic Compounds Assay Kit (Colorimetric)

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Phenolic Compounds Assay Kit (Colorimetric) (ab273293) provides a quick, sensitive and selective method for measuring the total amount of phenolic compounds in various biological samples.
Key facts
Detection method:Colorimetric,
Sample types:Food samples, Drinks, Natural/Herbal Products,
Assay type:Quantitative,
Sensitivity:= 0.02 mM,
Assay Platform:Microplate

Product details:
Phenolic Compounds Assay Kit (Colorimetric) (ab273293) provides a quick, sensitive and selective method for measuring the total amount of phenolic compounds in various biological samples. In this assay, phenolic compounds couple with diazonium salts under alkaline conditions to form a stable diazo chromophore, detectable by absorbance at 480 nm. Unlike the classical Folin-Ciocalteu (FC) protocol for measuring phenolic compounds, our assay is not affected by non-phenolic reducing substances such as sulfites, reducing sugars or ascorbic acid. The assay is high-throughput adaptable and can detect concentrations of phenolic compounds as low as 0.02 mM catechin equivalents (CEs) from a variety of plant and food-based samples.
Other Notes
This product was previously called K527 Biovision Phenolic Compounds Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phenolic compounds are a diverse group of secondary metabolites commonly found in plants. These compounds typically contain hydroxyl groups attached to aromatic rings. They perform a variety of mechanical roles within the cell including UV protection physical support and pigmentation. The aromatic rings enable phenolic compounds to participate in hydrogen bonding and metal chelation. These compounds have a molecular mass range of approximately 94 to over 300 daltons depending on the specific structure. Phenolic compounds occur ubiquitously in different parts of plants from fruits and seeds to roots and leaves.
Biological function summary
Phenolic compounds contribute significantly to the plant defense system. They act as antioxidants neutralizing free radicals and reducing oxidative stress. This antioxidative function is important in protecting cells from oxidative damage. Phenolic compounds such as flavonoids and tannins usually operate within complexes interacting with proteins and other biomolecules to strengthen plant defenses. These interactions affect various biological functions from growth regulation to response against pathogens.
Pathways
Phenolic compounds participate actively in the shikimic acid and phenylpropanoid pathways. The shikimic acid pathway is essential for producing aromatic amino acids serving as precursors for diverse phenolic compounds. The phenylpropanoid pathway branches into the flavonoid biosynthesis pathway which generates compounds involved in UV protection and pigmentation. These pathways also feature enzymes like phenylalanine ammonia-lyase which transforms phenylalanine into trans-cinnamic acid a critical phenolic compound precursor.
Phenolic compounds have been linked to cardiovascular disease and neurodegenerative disorders. Their antioxidant properties play a role in reducing oxidative damage associated with these conditions. In cardiovascular disease phenolic compounds like resveratrol show promise in mitigating oxidative stress-related damage. In the context of neurodegenerative disorders they help protect against neurotoxic compounds and maintain neuronal health. Through these actions phenolic compounds interact with proteins such as enzymes in oxidative stress regulation highlighting their beneficial effects against certain diseases.