Abcam, ab273617, Human Tau ELISA Kit

Size: 1 x 96Tests
Human Tau ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Tau with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Serum, EDTA Plasma, Cerebral Spinal Fluid,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 16 pg/mL,
Range:40.63 - 2600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Tau ELISA Kit ab273617 is a rapid single-wash 90-min ELISA kit to measure Human Tau in cerebral spinal fluid (CSF) cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 16 pg/mL.
How the assay works
Human Tau SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Tau protein in serum, plasma (EDTA and heparin), cell culture supernatant, cerebrospinal fluid, cell and tissue extract samples only. Urine, milk, saliva samples have not been tested with this kit. This kit is incompatible with plasma (citrate) samples.
The kit is 100% reactive with human recombinant Tau-A isoform and 61% reactive with human recombinant Tau-F isoform.
Recombinant human UCHL-1 and alpha synuclein were prepared at 7 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant human UCHL-1 and alpha synuclein were prepared at 50 ng/mL and 2 ng/mL and tested for interference with 2 ng/mL of recombinant human Tau. No interference with was observed.
Human Tau ELISA Kit ab273617 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Tau ELISA kit
ab237617
Human Tau ELISA kit
ab237617
has been used in the study of microglial surveillance in neurodegenerative diseases
Reference:
Dayuan Wang et al. 2022, PMID:36873190
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Mouse Tau Antibody Pair - BSA and Azide free
ab253413
- Anti-Tau antibody [EPR22524-211] - BSA and Azide free (Capture)
ab259552
- Anti-Tau antibody [EPR22521-17] - BSA and Azide free (Detector)
ab259658
Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.