Abcam, ab273699, Human ITGA6 (Integrin alpha 6) knockout SW480 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ITGA6 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:SW480,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ITGA6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Integrin alpha 6a often referred to as ITGA6 is a part of the integrin protein family. This protein acts mechanically as a receptor that binds extracellular matrix components. It functions mainly in cell adhesion and signal transduction processes. Integrin alpha 6a typically forms a dimer with the beta 1 or beta 4 subunit. This protein has a mass of approximately 130 kDa. Researchers observe its expression in epithelial cells endothelial cells and some immune cells throughout various tissues.
Biological function summary
Integrin alpha 6a plays a significant role in cell-matrix interactions by binding to laminin a major component of the basement membrane. It is part of a larger integrin complex known to affect cellular processes such as migration proliferation and differentiation. By mediating these interactions Integrin alpha 6a influences the structural stability of tissues and facilitates responses to cellular environment changes.
Pathways
Integrin alpha 6a participates in the MAPK and PI3K/Akt signaling pathways. These pathways are important for various cellular processes including growth survival and differentiation. Integrin alpha 6a associates with related integrin proteins such as Integrin beta 1 in these pathways where it modulates downstream signaling cascades that regulate cellular behavior and responses.
Integrin alpha 6a has associations with cancers and epidermolysis bullosa. Its expression correlates with tumor progression and metastasis particularly in cancers like breast and prostate. Integrin alpha 6a's involvement in epidermolysis bullosa links it to defects in epithelial-dermal cohesion. In these contexts its interaction with laminin and other integrins contributes significantly to pathogenesis and progression of these conditions.