Abcam, ab274392, Human TFPI ELISA Kit

Size: 1 x 96Tests
Human TFPI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TFPI with a sensitivity of 2.14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.14 pg/mL,
Range:12.5 - 800 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TFPI ELISA kit (ab274392) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFPI protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFPI with 21.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tissue factor pathway inhibitor (TFPI), also known as extrinsic pathway inhibitor (EPI) or Lipoprotein-associated coagulation inhibitor (LACI), inhibits coagulation through the formation of a complex with coagulation factor X, VII, and tissue factor (TF). TFPI functions to prevent the activation of factor X which thus preventing clot formation. Active TFPI is a 40kDa full-length protein is found 50% in LDL, 40% in LHDL, and 10% in VLDL. Abnormally high levels of TFPI are thought to lead to severe hemostatic disturbances such as thrombosis, disseminated intravascular coagulation, and septic shock. Similarly, TFPI is also used clinically to treat inflammatory and cardiovascular diseases where clot formation is a high prevalence.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C