Abcam, ab274394, Human CD163 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human CD163 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD163 with a sensitivity of 28.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 28.2 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CD163 ELISA Kit ab274394 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD163 in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 28.2 pg/mL.
How the assay works
Human CD163 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CD163 ELISA Kit ab274394 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Human CD163 ELISA Kit only requires a maximum of 12.5µL sample
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CD163 antibody [EPR22675-13] - BSA and Azide free (Capture)
ab274441
- Anti-CD163 antibody [EPR22675-102] - BSA and Azide free (Detector)
ab274441
CD163 is a scavenge receptor for the haptoglobin-hemoglobin complex. CD163 clears the complex in the liver, spleen and circulation. Soluble CD163 also plays a role in reducing inflammation, and dysregulation of CD163 is associated with some inflammatory conditions like multiple sclerosis, rheumatoid arthritis and diabetes mellitus.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary
CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.
Pathways
CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.
CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.