Abcam, ab274397, Human Erythropoietin ELISA Kit (EPO)

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human Erythropoietin ELISA Kit (EPO) is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human Erythropoietin (EPO) with a sensitivity of 1 mIU/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput - Need to design your own assay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1 mIU/mL,
Range:3.125 - 200 mIU/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Erythropoietin ELISA Kit (EPO) ab274397 is a rapid single-wash 90-min ELISA kit to measure Human Erythropoietin (EPO) in cell culture supernatant, Serum and plasma. This SimpleStep ELISA® sensitivity is 1 mIU/mL.
How the assay works
Human Erythropoietin (EPO) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human Erythropoietin. The standard protein in this kit is mature full length human Erythropoietin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin),and cell culture supernatant sample types. Spiked protein experiments were used to validate serum, plasma (citrate), plasma (EDTA), and plasma (heparin) sample types. Saliva, urine, milk, CSF, and cell and tissue extract samples have not been tested with this kit.
Human Erythropoietin ELISA Kit (EPO) ab274397 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
Save your precious samples
Our 384-well Human Erythropoietin ELISA Kit (EPO) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- EPO Antibody Pair - BSA and Azide free
ab253419
Related and recommended products
Human Erythropoietin ELISA Kit ab274397 is commonly used to study blood conditions such as anemia and polycythemia vera. Erythropoietin (EPO) plays a major role in the production of red blood cells.
Other ELISA kits to measure blood cell mechanisms and disorders are:
- Human Von Willebrand Factor ELISA kit
ab223864
- Human ICAM1 ELISA kit (CD54)
ab174445
- Human Leptin ELISA kit
ab179884
Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Human Erythropoietin has 83% amino acids sequence identity to rat Erythropoietin, and 80% identity to the mouse Erythropoietin.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C