Abcam, ab274399, Human CTHRC1 ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests
Human CTHRC1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CTHRC1 with a sensitivity of 27.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 27.3 pg/mL,
Range:93.75 - 6000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CTHRC1 ELISA Kit ab274399 is a rapid single-wash 90-min Sandwich ELISA to measure Human CTHRC1 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 27.3 pg/mL.
How the assay works
Human CTHRC1 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CTHRC1 ELISA Kit ab274399 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Human CTHRC1 ELISA Kit only requires a maximum of 12.5µL sample
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CTHRC1 antibody [EPR22851-144] - BSA and Azide free (Capture)
ab274447
- Anti-CTHRC1 antibody [EPR22851-215] - BSA and Azide free (Detector)
ab274447
Collagen triple helix repeat containing protein 1 (CTHRC1) is a 30 kDa secreted glycoprotein encoded by the
CTHRC1
gene. CTHRC1 is known to regulate extracellular collagen deposition and partake in vascular remodeling of arterial injury as a function of cellular response through the TGF-? and Wnt pathways. Moreover, mutation of the gene has been associated with carcinogenesis in a variety of cell and tissues, most notably esophageal adenocarcinoma and Barrett's esophagus. Furthermore, recent studies have demonstrated CTHRC1 as a marker of the severity of the disease progression in the dystrophic muscles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CTHRC1 protein also known as Collagen Triple Helix Repeat Containing 1 is a secreted protein with a mass of approximately 28 kDa. It plays a mechanical role in the extracellular matrix by modulating collagen fibrillogenesis and cross-linking. Increased expression of CTHRC1 protein often appears in areas undergoing active remodeling like those around wound healing sites fibrotic lesions and certain tumor tissues. The expression is not uniform across tissues and often corresponds to areas requiring structural reorganization or repair.
Biological function summary
CTHRC1 impacts cell migration and invasion. It associates with several extracellular matrix components influencing cellular response through cytoskeletal reorganization. There is no evidence for its participation as a part of a larger protein complex. The modulation of collagen interactions by CTHRC1 enables it to play a significant role in angiogenesis and tissue repair processes affecting endothelial and fibroblast cell functions.
Pathways
CTHRC1 participates in signaling pathways related to Transforming Growth Factor-beta (TGF-?) and Wnt. In the TGF-? pathway it impacts cellular responses to growth signals enhancing remodeling and repair mechanisms. Through the Wnt signaling pathway CTHRC1 interacts with Dishevelled proteins assisting in regulation and pathway activation related to cell proliferation and migration. These pathways highlight its involvement in important biological processes that influence tissue structure and cell communication.
CTHRC1 has relevance to both cancer progression and fibrotic diseases. Its overexpression is often noted in breast cancer where it drives tumor growth and metastasis interacting with other matrix components. Similarly in fibrosis CTHRC1 contributes to excessive collagen deposition and tissue stiffness. Proteins like TGF-? intersect with CTHRC1 functions further modulating disease pathway outcomes. Understanding CTHRC1's role in these disorders could inform therapeutic targets for modulating matrix dynamics.