Abcam, ab274951, Human PRMT2 knockout MCF7 cell line

Size: 1000000Cells / vial
PRMT2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 2 bp deletion after His125 of the WT protein Frameshift = 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:MCF7,
Species or organism:Human,
Tissue:Breast,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 2 bp deletion after His125 of the WT protein Frameshift = 100%,
Disease:Adenocarcinoma

Product details:
Recommended control:
Human wild-type MCF7 cell line (ab271144). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRMT2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Arginine Methyltransferase 2 (PRMT2) also known as HMT1 is a member of the protein arginine methyltransferase family. This enzyme carries out the transfer of methyl groups to arginine residues on target proteins influencing gene expression. PRMT2 has a molecular mass of about 59 kDa. It is detected widely in human tissues most notably in the liver heart and brain. This distribution indicates its involvement in a broad range of cellular activities.
Biological function summary
PRMT2 regulates various processes through its methylation activity. It functions as a coactivator particularly in the nuclear hormone receptor pathway. This protein is part of complexes that modify chromatin affecting gene transcription regulation. Through methylation of histones and other proteins PRMT2 influences cellular differentiation and proliferation. By modifying these key components PRMT2 controls important physiological functions within cells.
Pathways
PRMT2 interacts closely with nuclear hormone receptor signaling and pathways associated with gene regulation. These connections make it particularly important in the estrogen receptor signaling pathway where PRMT2 acts in conjunction with other proteins like PRMT1. Additionally PRMT2 has roles in influencing the PI3K/AKT pathway an important signaling pathway related to cell survival and metabolism.
PRMT2 exhibits relevant associations with conditions like cancer and neurological disorders. In cancer altered PRMT2 expression can misregulate gene expression through disrupted methylation promoting tumorigenic processes. Its dysregulation also connects to inflammation and degenerative diseases in the nervous system often involving proteins like PRMT1 as collaborators in pathology development. Understanding these links with diseases can guide therapeutic approaches involving PRMT2 modulation.