Abcam, ab275018, Exosome Panel (Calnexin, CD9, CD63, CD81, Hsp70, TSG101)

Size: 1Kit
Exosome Panel containing 6 antibodies against Calnexin, CD9, CD63, CD81, Hsp70, TSG101 - Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
Target:CANXSee target data

Product details:
Product Specifications
Exosome Panel (Calnexin, CD9, CD63, CD81, Hsp70, TSG101) (ab275018) is a panel of 6 rabbit recombinant monoclonal antibodies specific to exosome markers. Exosomes are small extracellular vesicles involved in cell communication, biomolecule transport and disease processes.
This panel contains antibodies to Calnexin (UniProt: P27824), CD9 (UniProt: P21926), CD63 (UniProt: P08962), CD81 (UniProt: P60033), Hsp70 (UniProt: P17879) and TSG101 (UniProt: Q99816) in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:
Rabbit recombinant monoclonal anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (
ab133615
Rabbit recombinant monoclonal anti-CD9 antibody [EPR23105-125] (
ab263019
Rabbit recombinant monoclonal anti-CD63 antibody [EPR5702] - Late Endosome Marker (
ab134045
Rabbit recombinant monoclonal anti-CD81 antibody [EPR21916] (20µL) (
ab219209
Rabbit recombinant monoclonal anti-Hsp70 antibody [EPR16892] (
ab181606
Rabbit recombinant monoclonal anti-TSG101 antibody [EPR7130(B)] (
ab125011
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 50 citations in peer-reviewed journals and are trusted by scientific community.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSG101 Calnexin CD9 CD63 CD81 and Hsp70 each have distinct mechanical roles in the cell. TSG101 also known as Tumor Susceptibility Gene 101 is a 46 kDa protein that plays an important role in endosomal sorting and is present in many tissues. Calnexin is a 90 kDa protein found in the endoplasmic reticulum involved in protein folding and quality control. The tetraspanins CD9 CD63 and CD81 are present on various cell surfaces with CD63 often found in late endosomes and lysosomes. CD9 and CD81 are smaller approximately 24-26 kDa. Hsp70 a heat shock protein of about 70 kDa functions as a molecular chaperone and is expressed under stress conditions in most cells.
Biological function summary
These proteins are involved in several essential cellular functions. TSG101 is part of the ESCRT-I complex which is important for membrane budding processes such as multivesicular body (MVB) formation and virus budding. Calnexin plays a role in the calnexin cycle ensuring proper protein folding in conjunction with Hsp70 which helps in protein stability and refolding damaged proteins during stress. CD9 CD63 and CD81 serve as exosome markers influencing exosome biogenesis and cargo sorting. They also play important roles in cell fusion migration and adhesion through interactions with integrins and other surface proteins.
Pathways
These proteins participate in key biological processes. TSG101 operates in the endocytic pathway marking proteins for degradation or recycling within the lysosome; it works alongside ubiquitin-related processes. Calnexin assists in the ER-associated degradation pathway maintaining protein homeostasis. CD9 CD63 and CD81 are integral in the extracellular vesicle pathway facilitating the transport of proteins and RNA. They also interact with integrins and other tetraspanins impacting cellular communication and immune response. Hsp70 supports these pathways by managing protein stability and cellular stress responses.
Abnormal expression of these proteins can lead to significant health issues. TSG101 mutations have links to cancer particularly breast cancer where overexpressed CD63 an exosome marker plays a role in tumor metastasis. Defects in Calnexin can result in glycoprotein misfolding and are associated with neurodegenerative diseases like Alzheimer's. Additionally CD9 is often implicated in fertility problems due to its involvement in gamete fusion. Hsp70 through its stress response role is relevant in neurodegenerative disorders where it helps mitigate protein aggregation. These connections highlight their importance in disease pathways and potential as therapeutic targets.