Abcam, ab27543, Anti-Apolipoprotein E3 antibody

Size: 100µg
Rabbit Polyclonal Apolipoprotein E antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human APOE.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human APOE.P02649,
Specificity:Ab27543 recognises Apolipoprotein EIII and cross reacts with Apolipoprotein EII and Apolipoprotein EIV.

Product details:
Although some customers have been successful in IHC we no longer batch test in this application.

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in water, Purification technique-Affinity purification Immunogen, Purification notes-Ab27543 was purified by affinity chromatography employing immobilized matrix., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E3 also known as ApoE3 or Apo E3 is an important molecule in lipid metabolism. The protein has a molecular weight of approximately 34 kDa. It is mainly expressed in the liver though significant amounts are produced by astrocytes and microglia in the central nervous system. ApoE3 is one of three major isoforms of apolipoprotein E and it plays an important role in the transport and clearance of lipids especially cholesterol by binding to lipoproteins and cellular receptors.
Biological function summary
The protein participates in lipid transport and homeostasis. ApoE3 binds to lipoprotein particles forming a complex that is essential for the proper transport of lipids in the bloodstream. This binding mechanism facilitates the uptake of lipoprotein particles by cells through receptor-mediated endocytosis. ApoE3 interacts closely with other lipid-associated proteins and receptor molecules enabling the efficient delivery of cholesterol and phospholipids to many tissues which is critical for maintaining cellular membrane integrity and other lipid-based biological functions.
Pathways
ApoE3 is an important player in the cholesterol metabolism and reverse cholesterol transport pathways. The protein interacts with LDL and HDL receptors which regulate cholesterol levels in the plasma. ApoE3's role in the hepatic clearance of chylomicrons and other lipoprotein remnants is significant for maintaining lipid balance. Additionally it cooperates with proteins like LDLR (Low-Density Lipoprotein Receptor) and LRP1 (LDL Receptor Related Protein 1) in orchestrating these pathways.
Research has shown that ApoE3 is less associated with adverse conditions than other isoforms like ApoE4. However disruptions in ApoE3 function or expression can contribute to dyslipidemia and cardiovascular diseases. In Alzheimer's disease the protein's isoforms particularly ApoE4 have varying implications but understanding ApoE3's interactions can provide insights into lipid-related pathology. ApoE3's connection with LDLR and its modulation of cholesterol levels underline its potential therapeutic role in managing such disorders.