Abcam, ab275900, Human Cleaved Caspase-7 (Asp198) ELISA Kit

Size: 1 x 96Tests
Human Cleaved Caspase-7 (Asp198) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cleaved Caspase-7 (Asp198) in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 21 pg/mL,
Range:281.25 - 18000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Cleaved Caspase-7 (Asp198) ELISA kit (ab275900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Caspase-7 protein in human cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cleaved Caspase-7 (Asp198) with 21 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Caspase-7 is a cytoplasmic cysteine protease involved in the activation cascade of caspases responsible for execution of apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs). Caspase-7 cleaves poly(ADP-ribose) polymerase (PARP). Caspase-7 overexpression promotes programmed cell death. Caspase-7 is expressed as an inactive pro-form (pro caspase-7). In apoptosis, the pro caspase-7 is activated by proteolytic cleavages at Asp23-Ala24, Asp198-Ser199 and Asp206-Ala207 bonds catalyzed by granzyme B and caspase-10, generating two subunits p20 and p11 that are assembled into hetero-tetrameric active enzyme. This kit measures Caspase-7 only upon cleavage of Asp198-Ser199 bond targeting the p20 subunit.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C