Abcam, ab275904, Human PAEP ELISA Kit (Glycodelin)

Size: 1 x 96Tests
Human PAEP ELISA Kit (Glycodelin) is a single-wash 90-min Simplestep used to quantify Human PAEP (Glycodelin) with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 3 pg/mL,
Range:7.81 - 500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human PAEP ELISA kit (ab275904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAEP protein in human serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAEP with 3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PAEP, progestogen-associated endometrial protein also known as glycodelin, is a glycoprotein which plays many important roles in fertility and maintenance of pregnancy. PAEP changes its biochemical interaction based on its glycosylation state. PAEP is a prognostic marker for in vitro fertilization and for premature membrane rupture. The antibodies in this kit were raised against a recombinant full-length mature protein. This protein does not have a homolog in mouse or rat and is not expected to cross-react with samples from these species.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C