Abcam, ab275905, Human LYST ELISA Kit

Size: 1 x 96Tests
Human LYST ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LYST with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 20 pg/mL,
Range:156.25 - 10000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human LYST ELISA kit (ab275905) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LYST protein in human cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LYST with 20 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
LYST, lysosomal trafficking regulator, is a vesicular transport protein. Defects in this protein lead to Chédiak–Higashi syndrome, a lysosomal storage disease that manifests as hypopigmentation, neutropenia, defective white blood cell function, and coagulation defects. The immunogen from which this kit was made represents the BEACH domain of the protein. Although this domain is conserved across many mammalian species, no cross-reactivity was seen.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LYST protein also known as Lysosomal Trafficking Regulator or Beige is involved in intracellular transport processes. With an approximate molecular mass of 429 kDa it plays a role in the trafficking of lysosomal contents. Expressed in various tissues it particularly affects those with a high demand for lysosomal activity such as immune system cells. As part of its mechanical function LYST regulates the morphology of lysosomes and endosomes by facilitating fusion and movement of these vesicles.
Biological function summary
LYST directly influences the cellular degradation and recycling process. The protein does not act alone but functions as part of a larger cellular machinery that maintains lysosomal homeostasis. Its role is particularly important in immune cell function where it ensures proper lysosomal delivery and secretion especially in cytotoxic T cells and natural killer cells. These activities are important for granulocyte and lymphocyte regulation ensuring a proper response to infections and cellular stress.
Pathways
The protein LYST is integral in pathways related to vesicular transport such as the endocytic and autophagic pathways. It has a well-documented interaction with proteins such as Rab7 which is important for endosome-to-lysosome trafficking. Additionally LYST interacts with components of the cytoskeleton which further influences lysosome positioning within the cell therefore supporting its central role in cellular logistic pathways.
Mutations or functional disruptions of the LYST protein are linked to Chediak-Higashi Syndrome a rare genetic disorder characterized by immunodeficiency bleeding issues and neurological problems. This condition demonstrates a connection between LYST and the immune response as well as interactions with proteins like Perforin which are also involved in immune functions. Moreover LYST alterations might contribute to pigmentary anomalies in certain skin disorders due to its involvement in melanosome biogenesis.