Abcam, ab277105, Anti-Ube2B antibody [PCRP-UBE2B-1C7]

Size: 100µg
Mouse Monoclonal UBE2B antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBE2B.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PCRP-UBE2B-1C7,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:Protein Array, ICC, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human UBE2B.P63146

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from bioreactor concentrate, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ube2B also known as ubiquitin-conjugating enzyme E2 B plays a mechanical role in the ubiquitination process. This protein carries out its function by transferring ubiquitin from an E1 enzyme to target substrates often assisted by an E3 ligase. Ube2B is a smaller protein with a mass of around 22 kDa. It shows expression in a wide range of tissues including testis and brain suggesting tissue-specific roles.
Biological function summary
The activity of Ube2B is central to the ubiquitin-proteasome pathway where it marks proteins for degradation. Ube2B operates as part of the larger ubiquitination cascade and interacts with various E3 ligases such as RNF8 which helps to mediate its target protein selectivity. This highly regulated process ensures proper cellular protein balance and is critical for maintaining cellular homeostasis.
Pathways
Ube2B has important roles in the DNA damage response and cell cycle regulation. The ubiquitin-proteasome system facilitated by Ube2B regulates the turnover of key cell cycle proteins and supports timely progression through cell cycle phases. Ube2B works closely with proteins such as RAD18 during DNA repair processes facilitating the post-replication repair of DNA lesions.
The altered function or expression of Ube2B impacts specific conditions such as cancer and neurodegenerative diseases. In cancer dysregulation can lead to uncontrolled cell proliferation due to abnormal degradation of regulatory proteins. Similarly neurodegenerative disorders involve Ube2B through aggregation and clearance failures of proteins like tau or alpha-synuclein. Ube2B's interaction with these disease processes accentuates its significance in maintaining cellular health.