Abcam, ab277248, Anti-Epiregulin antibody [EPR22373-68] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Epiregulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22373-68,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab277248 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Epiregulin. The recommended pair for sandwich ELISA is:
Capture:
ab277247
, Human Epiregulin Capture Antibody (unconjugated)
Detector: ab277248, Human Epiregulin Detector Antibody (unconjugated)
The reference range value is 6.25 - 400 pg/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Epiregulin also known as EREG is a member of the epidermal growth factor (EGF) family. It is a glycoprotein with a molecular weight of approximately 19 kDa. The protein is secreted and functions as a ligand for the EGF receptor (EGFR) and to a lesser extent for ErbB4 known as HER4 in humans. Epiregulin is expressed in a variety of tissues including placenta kidney and skin as well as within certain tumor cells. The expression can be regulated by signaling pathways involved in cellular growth and differentiation.
Biological function summary
Epiregulin participates in cellular processes such as proliferation differentiation and migration. It functions by binding to the EGFR initiating a cascade of downstream signaling events. As part of the EGF family it does not form part of a larger complex but it influences the activity of receptor dimers once bound. Its activity varies depending on the cellular context aiding in processes like tissue repair and embryonic development through its interaction with receptors and activation of signaling pathways.
Pathways
Epiregulin plays a significant role in the MAPK/ERK and PI3K/Akt signaling pathways both critical in regulating cell growth and survival. These pathways influence critical processes like cell cycle progression and apoptosis where epiregulin functions in coordination with other EGF family proteins such as TGF-alpha and amphiregulin. This protein assists in maintaining cellular homeostasis by modulating these pathways which are key to responding to environmental stimuli and cellular stress.
Epiregulin has associations with certain cancers such as colorectal and breast cancer. The protein often shows elevated expression levels in tumor samples implicating it in tumorigenesis. Its binding interaction with EGFR plays an important role in cancer cell survival and proliferation. Additionally epiregulin bears a connection to inflammatory diseases where its expression can exacerbate conditions like psoriasis through its impact on epidermal cells. In both cancer and inflammatory conditions the interplay between epiregulin and other related proteins like EGFR and ERBB2 influences disease progression and severity.