Abcam, ab277407, Mouse galectin 9/Gal-9 ELISA Kit

Size: 1 x 96Tests
Mouse galectin 9/Gal-9 ELISA Kit is a Quantitative ELISA for the measurement of Mouse galectin 9/Gal-9 in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Quantitative,
Sensitivity:= 0.18 ng/mL,
Range:0.164 - 40 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse galectin 9/Gal-9 ELISA kit (ab277407) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Mouse galectin 9/Gal-9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse galectin 9/Gal-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse galectin 9/Gal-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse galectin 9/Gal-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse galectin 9/Gal-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galectin-9 also known as Gal-9 or LGALS9 is a β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.
Biological function summary
Galectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.
Pathways
Galectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.
Galectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.