Abcam, ab277427, Human Plunc ELISA Kit

Size: 1 x 96Tests
Human Plunc ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Plunc in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 40 pg/mL,
Range:40.96 - 10000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Plunc ELISA Kit (ab277427) is an i
n-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Plunc in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Plunc coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Plunc present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Plunc antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Plunc bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Palate Lung Nasal Epithelium Clone (PLUNC) also known as SPLUNC1 or BPI-fold containing family A member 1 is a protein involved in host defense mechanisms. It has a molecular mass of approximately 25 kDa. High expression levels are found in the upper respiratory tract where it plays a role in airway surface liquid homeostasis. You can also detect it in salivary glands and other mucosal surfaces. Its expression is linked to innate immunity providing a first line of defense.
Biological function summary
This protein functions to modulate the physical and chemical environment at mucosal surfaces. It helps maintain the integrity of epithelial barriers against pathogens. As part of no particular protein complex PLUNC acts as a surfactant with anti-microbial properties in respiratory tissues. It helps maintain the viscosity and hydration of airway secretions facilitating the trapping and clearance of inhaled pathogens and particles.
Pathways
PLUNC is implicated in innate immune responses and mucosal defense mechanisms. It interacts within pathways involving the regulation of inflammation and mucociliary clearance. Proteins related to PLUNC in these pathways include SBEM and the airway-specific secretory proteins. Through these interactions it helps the lungs manage both pathogen load and airway clearances such as mucociliary escalator actions necessary to protect the respiratory system.
PLUNC is related to cystic fibrosis and chronic rhinosinusitis. In cystic fibrosis its regulation affects mucus viscosity and bacterial colonization potentially worsening respiratory symptoms. In chronic rhinosinusitis altered expression of PLUNC links to inflammation and recurrent infections. It connects with CFTR protein in cystic fibrosis impacting ion transport and mucociliary function. Through these mechanisms PLUNC can play a significant role in the progression of specific airway-related conditions.