Abcam, ab277431, Human CD229 ELISA Kit

Size: 1 x 96Tests
Human CD229 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD229 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 80 pg/mL,
Range:81.92 - 20000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human CD229 ELISA Kit (ab277431) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human CD229 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD229 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD229 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD229 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD229 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD229 also known as SLAMF3 or Ly9 is a transmembrane protein that weighs approximately 80 kDa. This protein belongs to the CD2 subset of the immunoglobulin superfamily. You can find high expression of CD229 in various lymphoid tissues such as the spleen thymus and peripheral blood lymphocytes. Its expression is notably high in T cells B cells and natural killer (NK) cells. As a homophilic receptor CD229 aids in cell-cell adhesion playing an essential role in immune response regulation among lymphocytes.
Biological function summary
CD229 influences signaling within immune cells. It functions as a mediator of adhesion and activation signaling pathways. CD229 does not act alone and often works as part of a receptor complex that may include CD48 and other members of the SLAM family. Besides its role in cellular adhesion CD229 participates in balancing immune activation and immune tolerance demonstrating its importance in developing and maintaining a robust immune response.
Pathways
CD229 participates in the adaptive immune signaling pathways. It links with other signaling proteins such as SAP (SLAM-associated protein) a critical modulator of downstream signaling cascades. SAP interacts with SLAM-family receptors including CD229 to regulate T and B cell activation and function. The involvement of CD229 in various immune signaling pathways positions it as a significant player in the broader context of cellular communication and response within the immune system.
CD229's aberrant expression or dysfunction links to autoimmune diseases such as systemic lupus erythematosus (SLE) and certain hematological malignancies. Altered CD229 expression has been observed in association with SLE where it may contribute to dysregulated immune responses. In hematological cancers CD229 overexpression or mutations can disrupt immune cell regulation promoting tumor progression. CD229 may interact with other SLAM-family proteins like CD244 which have implications in disease pathology and can be targets for therapeutic interventions.