Abcam, ab277532, Anti-SHANK2 antibody [EPR24142-35] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SHANK2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24142-35,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab277532 is the carrier-free version of
ab259966
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHANK2 also known as SH3 and multiple ankyrin repeat domains protein 2 is a scaffold protein of around 189 kDa. It is mainly expressed in the central nervous system especially in postsynaptic densities of dendritic spines in neurons. SHANK2 interacts with several synaptic proteins to organize the synaptic complexes that are important for neurotransmission. It helps in maintaining the structural integrity and signaling functions of synapses.
Biological function summary
SHANK2 provides key links in postsynaptic density structures. It interacts with other proteins like neuroligins and NMDA receptors to form a complex signaling network. This complex is involved in synaptic signal transduction which is critical for neural plasticity and communication between neurons. Through its role in synapses SHANK2 influences the strength and modulation of synaptic transmission.
Pathways
SHANK2 plays a significant role in neurotransmitter signaling pathways such as glutamatergic signaling. It directly interacts with proteins like Homer and mGluR that are critical to these pathways. These interactions influence synaptic growth and plasticity impacting cognitive processes like learning and memory. The proper functioning of these pathways depends on SHANK2's stability and interactions with other postsynaptic proteins.
Alterations in SHANK2 are linked to conditions like autism spectrum disorders and schizophrenia. Mutations in SHANK2 affect synaptic functions which are often seen in these neurological conditions. Additionally SHANK2 dysfunction relates to neuroligins and other postsynaptic proteins highlighting its involvement in the pathophysiology of these disorders. Understanding SHANK2's role can help in developing targeted therapies for such conditions.