Abcam, ab277792, Anti-PRMT5 antibody [3HCLC]

Size: 100µg
Rabbit Recombinant Multiclonal PRMT5 antibody. Suitable for ICC, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRMT5 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:3HCLC,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ChIP, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PRMT5 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.O14744

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRMT5 also known as Protein Arginine Methyltransferase 5 is an enzyme with molecular weight approximately 72 kDa. This protein catalyzes symmetric dimethylation of arginine residues on target proteins affecting gene expression and signal transduction. PRMT5 is expressed in various tissues with significant presence in the brain pancreas and lung. It enhances methyltransferase activity by interacting with other proteins and substrates.
Biological function summary
PRMT5 influences numerous cellular processes including RNA processing signal transduction and DNA repair. It functions as part of a protein complex often involving MEP50/WDR77 which modulates its methylating activity on histones and other proteins. This interaction dictates the cellular roles that are central to maintaining normal cellular function and regulation.
Pathways
PRMT5 operates within the Wnt/β-catenin signaling and mTOR pathways among others. As it modulates the methylation of transcription factors and regulators it contributes to transcriptional repression or activation which is important for cellular proliferation and differentiation. PRMT5 interacts with proteins such as Smad7 and cyclin D1 influencing these signaling pathways.
PRMT5 shows significant associations with cancer and neurological disorders. Elevated levels of PRMT5 correlate with the progression of various cancers including lung and breast cancers making it a potential target for therapeutic intervention. Additionally PRMT5's interaction with oncogenic proteins like MYC and MDM4 highlights its role in tumorigenesis. Furthermore PRMT5 dysregulation is linked to neurodegenerative diseases impacting the protein's target specificity and exacerbating disease symptoms.