Abcam, ab277797, Anti-Frizzled 4 antibody [22HCLC]

Size: 100µg
Rabbit Recombinant Multiclonal Frizzled 4 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Frizzled-4 aa 1-250.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:22HCLC,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Frizzled-4 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q9ULV1

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frizzled-4 also known as FZD4 is a protein important for the Wnt signaling pathway acting as a receptor for Wnt proteins. With a mass approximately 70 kDa FZD4 is a seven-transmembrane domain protein that is part of the G-protein-coupled receptor superfamily. Expression of FZD4 has been detected mostly in tissues like the retina and vasculature which suggests its role in the development and maintenance of these systems.
Biological function summary
Frizzled-4 plays a role in mediating signaling pathways that are essential for angiogenesis and retinal vascular development. It functions as a component of a larger receptor complex that includes co-receptors such as low-density lipoprotein receptor-related proteins (LRP5/6) and Disheveled proteins. This interaction is key for transmitting Wnt ligand signals to inside the cell influencing gene expression that drives cellular processes like proliferation and differentiation.
Pathways
The FZD4 protein is integral to the canonical and non-canonical Wnt signaling pathways. It actively participates in the β-catenin pathway which governs gene expression regulation by stabilizing β-catenin a process influencing various cellular activities. Moreover FZD4 is associated with the planar cell polarity pathway which is important for cell orientation processes. These pathways connect FZD4 to several other proteins like LRP5/6 and Disheveled which work in tandem to ensure proper signal transduction.
Mutations or dysregulation of Frizzled-4 are linked to familial exudative vitreoretinopathy (FEVR) a genetic disorder affecting retinal vascular development and leading to vision loss. The protein's interaction with LRP5 is significant in this context as mutations in either protein can disrupt their synergistic function resulting in the disease. Additionally aberrations in FZD4 signaling have implications in tumorigenesis particularly in cancers where Wnt signaling is pathologically activated suggesting that Frizzled-4’s role is important in maintaining tissue homeostasis.