Abcam, ab277800, Anti-Gli2 antibody [9HCLC]

Size: 100µg
Rabbit Recombinant Multiclonal GLI2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLI2 aa 1-350.
Key facts
Host species:Rabbit,
Clonality:Multiclonal,
Clone number:9HCLC,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GLI2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.P10070

Product details:
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
View our range of
recombinant multiclonal antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Gli2 also known as GLI family zinc finger 2 is a transcription factor with a molecular mass of approximately 160 kDa. It is an important component of the Hedgehog (Hh) signaling pathway. Gli2 primarily resides in the cytoplasm and migrates to the nucleus upon activation. Expression of Gli2 is widespread but most notable in developing tissues and adult brain. In the nucleus it regulates transcription by binding to specific DNA sequences.
Biological function summary
Gli2 acts as an activator or repressor of gene expression depending on the cellular context. It forms part of the Gli family of transcription factors playing a significant role in cellular growth differentiation and tissue patterning. While not usually part of stable protein complexes Gli2 interacts transiently with other proteins such as Suppressor of Fused (SuFu) to regulate its transcriptional activity. This interaction influences the processing and degradation of Gli proteins.
Pathways
The Hedgehog signaling cascade prominently features Gli2 as a critical mediator. This pathway is fundamental in embryonic development. Alongside other Gli proteins like Gli1 and Gli3 Gli2 helps to translate extracellular Hh signals into precise cellular responses. In the absence of Hh ligands Gli2 remains tethered in the cytoplasm in a complex with SuFu limiting its transcription activity. Upon Hh pathway activation Gli2 translocates to the nucleus and modulates target gene expression.
Gli2 has a notable connection to cancers including basal cell carcinoma and medulloblastoma. Overactive Hedgehog signaling often involves dysregulation of Gli2 which promotes tumorigenesis by driving inappropriate cellular proliferation. Additionally Gli2 links to the genetic disorder Greig cephalopolysyndactyly syndrome via its interactions with Gli3 protein. These connections underline the importance of Gli2 as a therapeutic target in certain pathological conditions.