Abcam, ab278087, Mouse GAS6 ELISA Kit

Size: 1 x 96Tests
Mouse GAS6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GAS6 with a sensitivity of 8.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.4 pg/mL,
Range:78.13 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse GAS6 ELISA kit (ab278087) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse GAS6 protein in mouse serum, plasma and cell culture supernatant.. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GAS6 with 8.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Gas6 (Growth arrest-specific protein 6), also known as AXL receptor tyrosine kinase ligand, is a secreted ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. Gas6 signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein Gas6 also known as growth arrest-specific 6 weighs approximately 75 kDa and functions mechanically as a ligand for the TAM (Tyro3 Axl and Mer) receptor tyrosine kinases. Gas6 protein facilitates cell signaling by binding especially to AXL receptor initiating various downstream cellular responses. It is widely expressed in different tissues including the nervous system cardiovascular system and kidneys. Additionally its expression increases in response to cellular stress and inflammatory stimuli.
Biological function summary
Gas6 influences several cellular processes such as cell survival proliferation and migration. Gas6 exerts its effects in a complex with AXL receptor which is part of a larger network involving other TAM receptors. By activating AXL Gas6 modulates immune responses inflammation and tissue repair. The protein also plays a significant role in phagocytosis helping remove apoptotic cells thereby maintaining tissue homeostasis.
Pathways
The Gas6 protein is significantly engaged in the PI3K/AKT signaling pathway important for promoting cell survival and inhibiting apoptosis. Additionally Gas6 interacts with the NF-kB pathway which regulates inflammation and immune responses. Both pathways highlight the synergy between Gas6 and AXL emphasizing their contribution to cellular integrity and response to injury.
Gas6's dysregulation associates with cancer and atherosclerosis. In oncogenesis altered Gas6-AXL signaling promotes tumor progression and resistance to therapy implicating it in diverse cancers. Meanwhile in cardiovascular diseases elevated Gas6 levels correlate with atherosclerosis interacting with inflammatory proteins such as interleukins. Understanding Gas6's role in these conditions offers potential for therapeutic targeting and biomarker development.