Abcam, ab278571, APC Anti-Syk (phospho Y352) + ZAP70 (phospho Y319) antibody [Zap70Y319-A3]

Size: 10Test / 100Test
Rabbit Recombinant Monoclonal ZAP70 phospho Y319 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZAP70 phospho Y319.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Zap70Y319-A3,
Isotype:IgG,
Light chain type:kappa,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human SYK phospho Y352. Database linkP43405

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Syk (spleen tyrosine kinase) and ZAP70 (zeta-chain-associated protein kinase 70) are non-receptor tyrosine kinases. Syk has a molecular mass of approximately 72 kDa while ZAP70 is around 70 kDa. They are expressed in hematopoietic tissues. Syk is found in B-cells and other myeloid lineages and ZAP70 is mainly located in T-cells and natural killer (NK) cells. These kinases play essential roles in signal transduction by phosphorylating proteins on tyrosine residues transmitting activation signals from receptors on the cell surface to the interior of the cell.
Biological function summary
Syk and ZAP70 play vital roles in the adaptive immune response. Syk is an essential mediator of B-cell receptor (BCR) signaling and assembles into a signaling complex with other proteins. ZAP70 functions similarly in T-cells becoming activated upon the engagement of the T-cell receptor (TCR). Both kinases help recruit and activate adaptor proteins and downstream signaling molecules that drive cellular responses including proliferation differentiation and survival.
Pathways
Both kinases are integral to the B-cell and T-cell receptor signaling pathways. Syk activates several pathways including the MAP kinase and NF-kB pathways through its interaction with proteins like BLNK and PLCγ2. ZAP70 initiates the TCR signaling cascade interfacing with LAT and SLP-76 to activate the same pathways. These pathways trigger transcriptional programs necessary for immune cell functions and development.
Syk and ZAP70 have connections to various immune-related conditions. Abnormal Syk activity relates to autoimmune diseases like rheumatoid arthritis where B-cell signaling plays a role. Syk interacts with proteins such as ITAM-containing immunoreceptors in these disorders. ZAP70 mutations are linked to immunodeficiency syndromes leading to dysfunctional T-cell signaling. These kinases make viable targets for therapeutic intervention bringing hope for better management of such immune diseases.